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Abstract #2306

Pattern of Mobile Lipid Accumulation in HeLa Cells During Paclitaxel-Induced Cell Death Using Diffusion-Weighted Spectroscopy.

Dominik Zietkowski1, Thomas R. Eykyn1, Geoffrey S. Payne1, Nandita M. deSouza1

1Cancer Research UK Clinical Magnetic Resonance Research Group, The Institute of Cancer Research, Sutton, Surrey, UK

Understanding the pattern of observed changes of mobile lipid resonances would help establish them as a biomarker of drug response. In this study, changes in mobile lipid resonances and their saturation following exposure of HeLa cells to the anti-mitotic drug paclitaxel were investigated using diffusion-weighted (DW) HR-MAS spectroscopy. It was observed that paclitaxel-induced apoptosis is associated with an increase of mobile lipid resonances (including unsaturated lipids) at 24h and a corresponding increase in lipid droplet formation. The pattern of changes in unsaturated lipids observed by 1H NMR may serve as a non-invasive time-course biomarker of apoptotic cell death.