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Abstract #2308

Hyperammonia and Hypoxia Induce Relevant Changes in Lipid Signals in 1H NMR Spectra from Human Cancer Cells

Sveva Grande1, Anna Maria Luciani1, Alessandra Palma1, Antonella Rosi1, Orazio Sapora2, Vincenza Viti1, Laura Guidoni1

1Dipartimento di Tecnologie e Salute and INFN, Gruppo Collegato Sanit, Istituto Superiore di Sanit, Roma, Italy, Italy; 2Dipartimento di Ambiente e connessa Prevenzione Primaria, Istituto Superiore di Sanit, Roma, Italy, Italy


Tumour tissues are often characterized by intense signals from fatty acids that have been widely studied to provide possible markers of tumour metabolism. We studied the effects of hyperammonia and hypoxia conditions in tumour cells, in order to provide more insight on these lipid metabolites and possible changes in their synthesis. Both hyperammonia and hypoxia induce significant increases in neutral lipid signals. Present data show that 1H NMR can be used to detect accumulation of neutral lipids due to conditions that are frequently encountered in tumour tissues pointing to a possible use as detectors of cell response to modified environment.

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