Abstract #2309

Mitochondrial Dysfunction Induces Mobile Lipids and Lipid Droplet Formation: Role of Lipid and Glucose Metabolism Monitored by 1H and 13C-Edited 1H MRS in Intact HuT 78 Lymphoblastoid Cells

Egidio Iorio¹, Claudia Testa², Francesco De Luca³, Cinzia Casieri⁴, Emanuela Lococo⁵, Roberto Carnevale⁵, Annarita Stringaro⁶, Maria Condello⁶, Giuseppe Arancia⁶, Luisa Lenti⁵, Luisa Lenti⁵, Roberto Strom⁷, Franca Podo¹

¹Cell Biology and Neurosciences, Istituto Superiore di Sanit, Roma, Italy; ²University of Bologna, Italy; ³Physics, University La Sapienza, Roma, Italy; ⁴University of L'Aquila, L'Aquila, Italy; ⁵Experimental Medicine and Pathology, University La Sapienza, Roma, Italy; ⁶Technology and Health, Istituto Superiore di Sanit, Rome, Italy; ⁷Cellular Biotechnologies and Haematology, University of La Sapienza, Rome, Italy

Several studies have reported the detection of mobile lipids (ML) signal in 1H MR spectra of intact cells under different physio-patological conditions. In this study we used a complex III inhibitor of mitochondrial electron transport antimycin A (AMC-A) in order to investigate the biochemical nature of ML formation and to monitor alterations of 13C label fluxes from [1-13C]-glucose in intact HuT 78 cells. We observed after 24h AMC-A treatment an over 10-fold increase in the ML signals and an incorporation of 13C label into ML signals. These results may open new perspectives to the development of non-invasive methods for in vivo monitoring the effects of mitochondrial impairment in a number of human diseases.