MRS Observed Effects of Phorbol Myristate Acetate on Lipid Metabolism in DU145 Prostate Tumor Cells

Melissa Suzanne Love¹, Daniel-Joseph Leung¹, Theresa Meganne Mawn¹, Edward James Delikatny¹

Phorbol myristate acetate (PMA) is a known activator of PKC and mimetic analogue of diacylglycerol, the cleavage product of PLC. We have investigated the effect of PKC activation by PMA on lipid metabolism in DU145 prostate tumor cells. Perfused DU145 cells were treated with PMA and analyzed with 31P MRS to measure levels of PC. Cultured DU145 cells were also treated with PMA and the phospholipase C inhibitor D609, and extracts were measured by high-resolution 1H MRS to examine levels of choline, PC, and GPC. The data shows that PMA affects lipid metabolism and has antiproliferative effects on DU145 cells.