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Abstract #2317

Cellular Glutathione Detected by 1H NMR Can Give Information on Radio-Sensitivity in Tumour Cells in Vitro

Alessandra Palma1, Claudio Giovannini2, Sveva Grande1, Laura Guidoni1, Anna Maria Luciani1, Antonella Rosi1, Orazio Sapora3, Vincenza Viti1

1Dipartimento di Tecnologie e Salute and INFN, Gruppo Collegato Sanit, Istituto Superiore di Sanit, Roma, Italy, Italy; 2Centro per la Qualit degli Alimenti e per i rischi Alimentari, Istituto Superiore di Sanit, Roma, Italy, Italy; 3Dipartimento di Ambiente e connessa Prevenzione Primaria, Istituto Superiore di Sanit, Roma, Italy, Italy

In previous studies we associated the glutathione (GSH) level -1H NMR detected- to radiation resistance. We then investigated whether i) such correlation holds also in tumour cells from human glioma in cell lines with different sensitivity to irradiation, and ii) a different role can be envisaged for GSH in cells from different tumours. Two adenocarcinoma-derived cell lines were therefore compared to two glioma-derived cell lines. These cell lines showed different radioresistance, consistent with the initial levels of GSH. Radiation resistant cells were then irradiated after Buthionine sulfoximine treatment to decrease the concentration of GSH in cells, preventing its radioprotective effects.