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Abstract #2318

The Nucleotide Sugar UDP-GlcNAc, a Major Glycosylation Precursor, Can Be Observed in Intact Tumour Cells by 1H NMR in the Low Field Spectral Region

Antonella Rosi1, Alessandra Palma1, Sveva Grande1, Laura Guidoni1, Anna Maria Luciani1, Vincenza Viti1

1Dipartimento di Tecnologie e Salute and INFN, Gruppo Collegato Sanit, Istituto Superiore di Sanit, Roma, Italy, Italy

Some aspects of sugar metabolism are involved in tumour progression. The dynamic changes in glycosylation levels in response to extracellular stimuli suggest a key role of signal transduction pathways. Nucleotide sugars have elicited much attention, being donor substrates for glycosylation of proteins and lipids.1H NMR was used to observe sugar-related metabolites in cells, in the low region of the spectrum. We observed major hexosamine precursors in two tumour cell lines. Modulation of signal intensities by NH4Cl treatment allowed to assign two nucleotide sugar signals, thus providing a tool for quantitation of these molecules in intact cells and, possibly, in tumour tissues.