Abstract #2319

Metabolic Characterization of an Imatinib-Resistant Chronic Myelogenous Leukemia Cell Model

Brian James Dewar¹, Kayvan Keshari¹, Rex Errol Jeffries¹, Lee M. Graves², Jeffrey M. Macdonald¹

¹Biomedical Engineering, Univ. of North Carolina, Chapel Hill, NC, USA; ²Pharmacology, Univ. of North Carolina, Chapel Hill, NC, USA

Drug resistance to Imatinib often develops in CML. Here we characterized metabolic differences using NMR, comparing an Imatinib-resistant cell model, MyLR, to a Imatinib-sensitive parental cell line, MyL. Here we describe the first such example of elevated phosphocreatine levels in drug -resistant leukemia cell using conventional MRS metabolomics and NMR compatible bioreactor.