Abstract #2320

Effect of Androgens on Intracellular Polyamine Levels in Androgen-Dependent and Androgen-Independent Prostate Cancer Cell Lines

Nicholas G. Spencer¹, Eszter Nagy², Nandita M. DeSouza¹, Geoffrey S. Payne¹

¹CRUK Clinical Magnetic Resonance Research Group, Institute of Cancer Research and the Royal Marsden Hospital, Sutton, Surrey, UK; ²Human Biomonitoring and Carcinogen Activation, Institute of Cancer Research and the Royal Marsden Hospital, Sutton, Surrey, UK

With the long-term aim to evaluate polyamines as magnetic resonance (MR) markers of androgen-sensitivity in prostate cancer, this study compares polyamine levels in androgen-dependent and androgen-independent cell lines in response to androgen deprivation. An androgen-responsive cell line, 22RV1, showed lower polyamine levels compared to androgen-independent PC-3 cells and, in androgen deprived medium, there was a decrease in polyamines for 22RV1 but not in PC-3 cells. This was confirmed with high performance liquid chromatography and demonstrates the potential of MR in the analysis of polyamine metabolism in-vitro models of prostate cancer.