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Abstract #2320

Effect of Androgens on Intracellular Polyamine Levels in Androgen-Dependent and Androgen-Independent Prostate Cancer Cell Lines

Nicholas G. Spencer1, Eszter Nagy2, Nandita M. DeSouza1, Geoffrey S. Payne1

1CRUK Clinical Magnetic Resonance Research Group, Institute of Cancer Research and the Royal Marsden Hospital, Sutton, Surrey, UK; 2Human Biomonitoring and Carcinogen Activation, Institute of Cancer Research and the Royal Marsden Hospital, Sutton, Surrey, UK


With the long-term aim to evaluate polyamines as magnetic resonance (MR) markers of androgen-sensitivity in prostate cancer, this study compares polyamine levels in androgen-dependent and androgen-independent cell lines in response to androgen deprivation. An androgen-responsive cell line, 22RV1, showed lower polyamine levels compared to androgen-independent PC-3 cells and, in androgen deprived medium, there was a decrease in polyamines for 22RV1 but not in PC-3 cells. This was confirmed with high performance liquid chromatography and demonstrates the potential of MR in the analysis of polyamine metabolism in-vitro models of prostate cancer.

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