Investigation of Features Related to Androgen Dependency in Prostate Cells Using 1H HRMAS MRS and Principal Component Analysis

Robert Leslie Davidson¹, Nick Spencer¹, Simon J. Doran¹, Nandita M. deSouza¹, Geoffrey S. Payne¹

Principal Components Analysis applied to 1H HRMAS MRS of normal and cancerous prostate cell lines grown with and without androgens to study the metabolic features characteristic of androgen independence. Preliminary studies show a large number of peaks that can discriminate between healthy and cancerous cell lines (P-Cho, Ala and Lac elevated in cancer, Cr and polyamines elevated in healthy cells), and also that creatine is less abundant in androgen insensitive cancer cell lines when grown in the absence of androgen.