Barbara Blasiak1,2, Boguslaw Tomanek1,3, Tadeusz Foniok3, David Kirk1, David Rushforth3, Roger MacKenzie4, Abedelnasser Abulrob4,5, Umar Iqbal5, Danica Stanimirovic4,5, Xuqueng Lung1, Peter Forsyth1
1Department of Clinical Neurosciences, University of Calgary, Calgary, Alberta, Canada; 2Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, malopolskie, Poland; 3Institute of Biodiagnostics(West), National Research Council of Canada, Calgary, Alberta, Canada; 4Institute of Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada; 5Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada
The composition of targeted contrast agents of gliomas was investigated. The c.a. consisting of Fe3O4 and FeCo cores with SiO2 and Au shells were investigated. The relaxation times of agar solutions of the c.a. were measured ex vivo using 9.4T MRI system. To render NPs targeted high grade glioma specific single domain antibodies were conjugated with the NPs. The ex vivo results showed, that the FeCo core are more efficient than Fe3O4 core NPs. The in vivo MRI using mouse glioma model and functionalized NPs showed decrease in T2 over the tumor area, showing the efficacy of the c.a.