Valeria Righi1,2, Dionyssios Mintzopoulos1,2, Yiorgos Apidianakis1,3, Ovidiu C. Andronesi1,2, Laurence G. Rahme1,3, A. Aria Tzika1,2
1NMR Surgical Laboratory, MGH & Shriners
Hospitals, Harvard Medical School, Boston, MA, USA; 2Radiology,
Athinoula A. Martinos Center for Biomedical Imaging, Boston, MA, USA; 3Molecular
Surgery Laboratory, MGH & Shriners Hospitals, Harvard Medical School,
Boston, MA, USA
We demonstrate biomarker profiles with high-resolution magic angle spinning proton MR spectroscopy (HRMAS H1 MRS) of live Drosophila melanogaster flies. Aged flies exhibited more lipids following trauma, indicating that older flies are susceptible to trauma-mediated lipid homeostatic perturbations. In contrast, even young but immunocompromised flies also exhibited increased lipids suggesting that trauma elicits age-dependent lipid homeostatic perturbation that may promote immune dysfunction. We propose for the first time that in vivo HRMAS H1 MRS of Drosophila melanogaster in a 14 tesla spectrometer is a feasible and valuable model system for spectroscopic investigations of trauma, innate immunity and aging.