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Abstract #2408

Metabolism of Hyperpolarized 1, 4-13C2-Fumarate in Human Cancer Cell Lines

Anna Gisselsson1, Magnus Kalsson1, Pernille Rose Jensen1, Ren in 't Zandt1, Georg Hansson1, Mathilde H. Lerche1

1Imagnia AB, Malm, Sweden

Hyperpolarization of metabolic markers provides a powerful tool to study real time metabolism on a cellular level. To investigate if hyperpolarized fumarate can be used as a marker to study an altered metabolism in cancer, we studied the conversion of 1,4-13C2-fumarate into 1,4-13C2-malate in four human cancer cell lines; breast adenocarcinoma (MDA-MB-231), chondrosarcoma (H EMC-SS) and two prostate cancer lines (PC-3 and DU-145). Conversion of 1,4-13C2-fumarate to 1,4-13C2-malate could be seen in all four cancer lines investigated. The observed difference in conversion rate into 1,4-13C2-malate between cell lines of varying aggressiveness opens up for a potential new method to stage cancer.