Abstract #3118

In Vivo MR Imaging of Renal Ischemia Reperfusion Injury in Mice Using Microparticles of Iron Oxide Targeting VCAM-1

Jurgen E. Schneider¹, Asim M. Akhtar¹, Hannah Barnes¹, Stephanie Baker², Janet E. Digby¹, Martina A. McAteer¹, Kathryn Wood², Robin P. Choudhury¹

¹Cardiovascular Medicine, University of Oxford, Oxford, Oxon, UK; ²Nuffield Department of Surgery, University of Oxford, Oxford, Oxon, UK

Ischemia/reperfusion injury (IRI) is an important cause of tissue damage in vascular syndromes of the heart, brain and kidney, but sensitive tools to image ischemic injury in vivo are lacking. Our study demonstrated that (a) antibody-conjugated microparticles of iron oxide (MPIO) targeting VCAM-1 enabled molecular magnetic resonance imaging of endothelial activation in mouse renal IRI; (b) the retained MPIO were readily quantifiable from the MR images. Our approach provides a platform for non-invasive detection of IRI in the kidney and potentially other organs.