Abstract #3601

Explanation of High Fractional Anisotropy Value in the Wall and Cavity of the Brain Abscess Differs as Evident by Histology and Immunohistochemistry

Rakesh K. Gupta¹, Sona Saksena¹, Nuzhat Husain², Mazhar Husain³, Savita Srivastava², Mahesh Ramola³, Rishi Awasthi¹, Kashi N. Prasad⁴, Ram K.S. Rathore⁵

¹Radiodiagnosis, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India; ²Pathology, Chhatrapati Shahuji Maharaj Medical University, Lucknow, Uttar Pradesh, India; ³Neurosurgery, Chhatrapati Shahuji Maharaj Medical University, Lucknow, Uttar Pradesh, India; ⁴Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India; ⁵Mathematics and Statistics, Indian Institute of Technology, Kanpur, Uttar Pradesh, India

DTI data were acquired from eight patients with brain abscess (BA) with an aim to see the difference in the relationship between intercellular cell adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) expression and fractional anisotropy (FA) in the BA wall and cavity and its possible explanation vis--vis histology. Quantitative immunohistochemical analysis was performed for ICAM-1 and LFA-1 in the wall showing maximal positive staining. Higher FA values in the wall compared to the cavity even when ICAM-1 and LFA-1 were not expressed in the collagen fibers suggest that the concentrically laid collagen fibers provide structural orientation and are responsible for increased FA. In the BA, there are different explanations for increased FA in cavity and wall substantiated by histology and immunohistochemistry.