Abstract #4141

Monitoring Kidney Viability Before Transplantation by Means of 31P CSI and Oxygenated Hypothermic Perfusion

Francois Lazeyras^1,2, Leo Buhler³, Jean-Paul Vallee¹, Antonio Nastasi³, Raphael Ruttimann³, Philippe Morel³, Jean-Bernard Buchs³

¹Service of Radiology, University of Geneva and University Hospital of Geneva, Geneva, Switzerland; ²Work supported in part by the Center for Biomedical Imaging (CIBM), Geneva and Lausanne, Switzerland; ³Visceral and Transplantation Service, University Hospital of Geneva, Geneva, Switzerland

The experimental studies presented in this paper show that ATP is resynthesized if kidneys are preserved with oxygenated hypothermic pulsatile perfusion (O2+HPP). The development of a MR compatible perfusion device allowed monitoring 31P spectra during continuous perfusion. Our results show that ATP reserve remains high if O2+HP is applied immediately after kidney harvesting. In this condition, PME remains elevated. In opposition, in absence of perfusion, gradual depletion of PME and limited resynthesis of ATP are observed. This technique may bring new insight for marginal organs reanimation and evaluation prior to transplantation.