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Abstract #4163

NMR Structural Characterization and Inhibition of Colon Cancer Cells by Components of Citrus Aurantium L.

G K. Jayaprakasha1, Jadegoud Yaligar2, G A. Nagana Gowda3, Bhimanagouda S. Patil1

1Horticultural Sciences, Vegetable & Fruit Improvement Center,Texas A&M University, College Station, Texas-77843, USA; 2Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, New York-10065, USA; 3Chemistry, Purdue University, West Lafayette, Indianapolis-47907, USA

Epidemiological data suggest that ingestion of some bioactive compounds from fruits and vegetables may contribute to reduction of cancer incidence in humans. Colon cancer is second leading cause of cancer deaths in US. Two bioactive compounds were isolated from sour oranges (Citrus aurantium L.) and their structure was unambiguously confirmed by 1D and 2D NMR analysis. Limonexic acid at 24h post treatment showed significant cytotoxic effect at low concentration as compared to untreated cells. However sitosterol glucoside showed significant cytotoxic effect only at 10.0 M(p<0.05) concentration. Both compounds have ability to arrest DNA synthesis and G2/M phases of cell cycle.