Abstract #4162

The Analysis of Lipid and Macromolecule Signals in HR-MAS Data Reveals Information on the Nature of Cytoplasmic Lipid Droplets

Martin Wilson^1,2, Greg M. Reynolds³, Risto A. Kauppinen⁴, Theo N. Arvanitis^2,3, Andrew C. Peet^1,2

¹Cancer Sciences, University of Birmingham, Birmingham, West Midlands, UK; ²Oncology, Birmingham Childrens Hospital Foundation Trust, Birmingham, West Midlands, UK; ³School of Electronic, Electrical & Computer Engineering, University of Birmingham, Birmingham, West Midlands, UK; ⁴Dartmouth Medial School, Dartmouth College, Hanover, NH, USA

A method is presented to investigate the signals originating from cytoplasmic lipid droplets (CLDs) and macromolecules present in 1H HR-MAS spectra. Data from a panel of 18 cell lines, derived from a range of childhood nervous system tumours, is analysed and strong correlations are found between lipid signals at 0.9. 1.3, 1.6 and 5.3ppm. Weaker correlations are also observed for the macromolecule peaks. The relative ratios between the lipid peaks are constant implying that CLD signals originate from a homogeneous group of species.