In Vitro Spectroscopic Method Under Clinical Conditions for in Vivo Mannitol Detection

Gabriela Hossu¹, Franois Kauffmann², Nicolas Courouble¹, Caroline Henry³, Mikael Jokic⁴, Antoine Coquerel⁵, Pierre Denise⁶, Jean-Marc Constans^1,7

Using prior knowledge for in vitro studies performed under the same conditions as patient examinations may improve identification of lesser-known components such as neurogenesis peaks, apoptosis biomarkers or medications. We studied in vitro measurements acquired under in vivo conditions using a test object composed of three separate compartments for Mannitol drug and using 1% TMS in CDCl3 and Choline as internal references. We propose a new approach to analyzing and validating reproducibility of spectroscopic patterns. Consequently, in vivo identification of Mannitol accumulation in human brain pathologies can be measured, for the first time, with a higher level of precision.