Solid-State NMR Adiabatic TOBSY Provides Enhanced Sensitivity for Multidimensional High-Resolution Magic-Angle-Spinning H1 MR Spectroscopy in Burn Trauma

Valeria Righi¹, Ovidiu C. Andronesi¹, Dionyssios Mintzopoulos¹, A. Aria Tzika¹

We propose a solid-state NMR method that maximizes the advantages of high-resolution magic-angle-spinning (HRMAS) applied to intact biopsies when compared to more conventional liquid-state NMR approaches. Numerical simulations and experimental results on skeletal muscle specimens from burn trauma are presented. Experimentally, it is shown that an optimized adiabatic TOBSY (TOtal through Bond correlation SpectroscopY) solid-state NMR pulse sequence for two-dimensional 1H-1H homonuclear scalar-coupling longitudinal isotropic mixing provides a 50-60% improvement in signal-to-noise ratio relative to its liquid-state analogue TOCSY (TOtal Correlation SpectroscopY). We demonstrate a concept for HRMAS metabolic profiling of burn trauma, from biopsies requiring reduced sample degradation for further genomic analysis.