Acute Liver Failure Studied by Hyperpolarized 1,4-13C2-Fumarate in CCl4 Injured Rat Liver

Rene in 't Zandt¹, Magnus Karlsson¹, Anna Gisselsson¹, Pernille Rose Jensen¹, Georg Hansson¹, Mathilde Lerche¹

We have developed a diagnostic marker - hyperpolarized 1,4-¹³C₂-fumarate, which takes advantage of the technique dynamic nuclear polarization for magnetic resonance imaging (DNP-MRI). This marker allows real-time metabolic studies of a TCA-cycle intermediate, ¹³C-malate. With this marker we have investigated the metabolism in the liver of CCl₄ treated rats.