1,4-13C2 Malate Reports on Ischemia Related Reperfusion Injury, After Administration of Hyperpolarized 1,4-13C2 Fumarate in Mouse Skeletal Muscle in Vivo

Pernille Rose Jensen¹, Rene in 't Zandt¹, Magnus Karlsson¹, Anna Gisselsson¹, Georg Hansson¹, Mathilde Hauge Lerche¹

Hyperpolarized 1-¹³C pyruvate has been used to visualize that the PDH flux is affected in the stunned myocardium in vivo and in vitro. Where metabolism of hyperpolarized pyruvate reports on ischemia related injury in the heart we have found that another metabolic marker, hyperpolarized 1,4-¹³C₂ fumarate may be responsive on longer time scales and report on reperfusion injury. Hyperpolarized 1,4-¹³C₂ fumarate was studied in an ischemic model in the resting mouse hind leg skeletal muscle and show the possible complementary value to pyruvate in reperfusion injury after an ischemic insult.