Meeting Banner
Abstract #0206

A Multimodality Investigation of the Dynamics, Trafficking and Properties of Iron Oxide Core High-Density Lipoprotein in Experimental Atherosclerosis

Torjus Skajaa1,2, David Peter Cormode1, Peter Jarzyna1, Courtney Blachford3, Amanda Delshad1, Edward A. Fisher3, Ronald E. Gordon4, Zahi A. Fayad1, Willem J.M Mulder1

1Translational and Molecular Imaging Institute, Mount Sinai School of Medicine, New York, NY, United States; 2Dept. of Cardiology, Clinical Institute, Aarhus University Hospital (Skejby), Aarhus, Denmark; 3School of Medicine, New York University, New York, NY, United States; 4Department of Pathology, Mount Sinai School of Medicine, New York, NY, United States

FeO-HDL is a lipoprotein derived nanoparticle platform detectable by MRI, optical imaging and TEM. In the current study FeO-HDL was synthesized, applied to various cell lines in vitro and to apoE-KO and wild type mice in vivo. Characterization of FeO-HDL revealed close resemblance to native HDL. In vitro experiments confirmed the aforementioned and showed excellent biocompatibility. Upon intravenous administration in vivo MRI experiments on apoE-KO mice revealed their uptake in the lesioned vessel wall, which was confirmed histologically. Lipid exchange measurements showed lipid transfer from FeO-HDL to native lipoproteins. Conclusively we have shown that FeO-HDl closely resembles native HDL.