Torjus Skajaa1,2, David Peter Cormode1,
Peter Jarzyna1, Courtney Blachford3, Amanda Delshad1,
Edward A. Fisher3, Ronald E. Gordon4, Zahi A. Fayad1,
Willem J.M Mulder1
1Translational and Molecular Imaging
Institute, Mount Sinai School of Medicine, New York, NY, United States; 2Dept.
of Cardiology, Clinical Institute, Aarhus University Hospital (Skejby),
Aarhus, Denmark; 3School of Medicine, New York University, New
York, NY, United States; 4Department of Pathology, Mount Sinai
School of Medicine, New York, NY, United States
FeO-HDL
is a lipoprotein derived nanoparticle platform detectable by MRI, optical
imaging and TEM. In the current study FeO-HDL was synthesized, applied to
various cell lines in vitro and to apoE-KO and wild type mice in vivo.
Characterization of FeO-HDL revealed close resemblance to native HDL. In
vitro experiments confirmed the aforementioned and showed excellent biocompatibility.
Upon intravenous administration in vivo MRI experiments on apoE-KO mice
revealed their uptake in the lesioned vessel wall, which was confirmed
histologically. Lipid exchange measurements showed lipid transfer from
FeO-HDL to native lipoproteins. Conclusively we have shown that FeO-HDl
closely resembles native HDL.