Meeting Banner
Abstract #0397

Hypoxic Environments Disrupt Collagen I Fibers and Macromolecular Transport

Samata Kakkad1, Marie-France Penet1, Meiyappan Solaiyappan1, Arvind Pathak1, Venu Raman1, Kristine Glunde1, Zaver M. Bhujwalla1

1JHU ICMIC Program, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United States

Solid tumors are characterized by hypoxic environments. Hypoxia stimulates the gene expression of a cluster of hydroxylases used for collagen fiber formation. Hypoxic environments in tumors may lead to abnormal collagen deposits either by cancer cells or by fibroblasts within the tumor stroma. In normal tissue collagen fibers direct interstitial fluid into lymphatic channels. In tumors these fibers may not be structured for efficient flow of fluid, especially in hypoxic areas. Our purpose was to understand the role of hypoxia in modifying macromolecular fluid transport using MRI, and collagen fiber distribution using second harmonic generation microscopy.