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Abstract #0568

Detecting Response to Treatment in Human Breast Adenocarcinoma Using a Co-Administration of Hyperpolarized [1-13C]pyruvate and [1,4-13C2]fumarate

Timothy H. Witney1,2, Mikko I. Kettunen1,2, De-en Hu1,2, Ferdia A. Gallagher1,2, Kevin M. Brindle1,2

1Department of Biochemistry, University of Cambridge, Cambridge, Cambridgeshire, United Kingdom; 2Cancer Research UK Cambridge Research Institute, Cambridge, Cambridgeshire, United Kingdom

In the current study, we used a co-administration of hyperpolarized [1-13C]pyruvate and [1,4-13C2]fumarate as a sensitive marker of cell death in a model of human breast adenocarcinoma following treatment with a DNA damaging agent. The results show that a decrease in pyruvate - lactate exchange coincides with the induction of cell death in breast cancer cells both in vitro and in vivo, with an increase in fumarate - malate exchange shown to correlate to the onset of necrosis.