Meeting Banner
Abstract #0752

Use of MRI for Longitudinal in Vivo Phenotyping of Obese Mouse Models Following a Dietary Intervention

Abdel Wahad Bidar1, Karolina Ploj2, Christopher Lelliott2, Karin Nelander3, Leonard Storlien2, Paul Hockings1

1DECS Imaging, AstraZeneca R&D, Mlndal, Sweden; 2CVGI, Bioscience, AstraZeneca R&D, Sweden; 3DECS Discovery Statistics, AstraZeneca R&D, Sweden

In preclinical drug discovery, experimental rodent models of obesity are used for the investigation of metabolic disorders. Repeated in vivo measurements of adipose tissue depots and intraorgan fat can provide longitudinal data with greatly reduced usage of experimental animals. The aim of the present study was threefold: (i) validate in vivo MRI/S determinations of brown adipose tissue, total, intra-abdominal and subcutaneous white adipose tissues as well as intrahepatocellular lipids against ex vivo measurement, (ii) address the 3Rs mandate, by presenting a statistical power analysis; (iii) characterize the phenotypic and metabolic switch of the cafeteria-diet mouse model during a dietary intervention.