Richard Jonkers1, Tom Geraedts1, Luc van Loon2, Klaas Nicolay1, Jeanine Prompers1
Insulin resistance and type 2 diabetes are associated with elevated liver lipid content. It remains unknown whether this excessive accumulation of triglycerides is a result of increased lipid uptake or decreased lipid oxidation. In this study, we measured for the first time postprandial lipid storage in rat liver in vivo using localized 13C-edited 1H-observed MRS and 13C labeled lipids as tracers. The 13C enrichment of the liver lipid pool was 0.9 0.7% at baseline and increased to 4.8 0.9% 5h after ingestion of the tracer, showing that we can assess changes in 13C enriched lipid content in vivo.