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Abstract #0878

In Vivo Assessment of Ca2+-Related Glucose Homeostasis in Skeletal Muscle Using Manganese-Enhanced Magnetic Resonance Imaging

Li-Wen Lee1,2, Po-Wah So3, Jimmy D. Bell1

1Metabolic and Molecular Imaging Group, MRC Clinical Sciences Centre, Hammersmith Hospital Campus, Imperial College London, London, United Kingdom; 2Department of Diagnostic Radiology, Chang Gung Memorial Hospital, Chang Gung Univeristy College of Medicine, Chiayi, Taiwan, Taiwan; 3Preclinical Imaging Unit, King's College London, Institute of Psychiatry, James Black Centre, Denmark Hill Campus, London, United Kingdom


Glucose homeostasis is regulated by β-cell insulin secretion, peripheral glucose uptake and hepatic glucose production. We applied manganese-enhanced magnetic resonance imaging (MEMRI), utilising Mn2+ as a surrogate marker for Ca2+, to assess Ca2+-related processes involved in glucose homeostasis. MEMRI was performed following i.p. glucose or vehicle and signal intensity (SI) measured to assess Mn2+ distribution. MEMRI showed a significant increase in SI in skeletal muscle and the pancreas following glucose challenge but not in the liver. Thus, MEMRI can be used to assess changes in skeletal muscle glucose uptake and β-cell insulin secretion in vivo.

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