Abstract #0914

Localized 31P Saturation Transfer in Rat Brain

Vladimir Mlynarik¹, Cristina Cudalbu¹, Yves Pilloud¹, Rolf Gruetter^1,2

¹Laboratory of Functional and Metabolic Imaging, Ecole Polytechnique Fdrale de Lausanne, Lausanne, Switzerland; ²Departments of Radiology, Universities of Lausanne and Geneva, Switzerland

Phosphorus saturation transfer technique is sensitive to experimental imperfections such as partial direct saturation of the measured peak, incomplete saturation of the other peak under exchange and a problematic localization, which is usually done by an active volume of a surface coil used as a transceiver. In our study we compared the PCr <> γ-ATP saturation transfer experiment using 1D ISIS localization combined or not combined with outer volume saturation. We observed a contaminating component from muscles in the PCr signal when using the 1D ISIS only. This contamination led to an underestimation of the calculated rate constant of the creatine kinase reaction.