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Abstract #1012

Metabolic Imaging of the Perfused Rat Heart Using Hyperpolarized [1-13C]Pyruvate

Philip Lee1, Marie Schroeder2, Daniel Ball2, Kieran Clarke2, George Radda1, Damian Tyler2

1Singapore Bioimaging Consortium, Biomedical Sciences Institute, Singapore, Singapore; 2Department of Physiology, Anatomy & Genetics, University of Oxford, United Kingdom

Imaging of cardiac metabolism using 13C-MRS is currently hindered by both a low sensitivity and a low natural abundance of 13C. The recent development of liquid state Dynamic Nuclear Polarization (DNP) techniques has dramatically increased the signal available from 13C-MRS experiments and has opened up new possibilities for metabolic imaging of the heart. By injecting hyperpolarized 13C-labeled pyruvate into a perfused rat heart, followed by high spatial resolution chemical shift imaging (CSI) during an optimum acquisition window, we were able to image the bio-distribution of lactate, bicarbonate and alanine within 46 s.