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Abstract #1013

Investigation of Hepatic Metabolism of DNP Hyperpolarized 1,4-13C2 Succinate

Jeremy W. Gordon1, Kang Wang1, Sean B. Fain1,2, Ian J. Rowland2

1Medical Physics, University of Wisconsin-Madison, Madison, WI, United States; 2Radiology, University of Wisconsin-Madison, Madison, WI, United States

This work describes the successful hyperpolarization of 1,4-13C2 succinate using dynamic nuclear polarization (DNP). By optimizing the solubility of succinic acid in aqueous solution, levels of solid state similar to that of pyruvate can be reproducibly obtained. 3.0M solutions of hyperpolarized succinate were utilized to investigate hepatic metabolism in vivo. 13C spectra and imaging show that the biodistribution of succinate within the liver is homogenous. No metabolites were observed in the time frame of the hyperpolarized experiments. Metabolites were also not observed in ex vivo organ homogenates.