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Abstract #1023

Metabolism of Hyperpolarized 1-13C-Lactate in Living Breast Cancer Cell Cultures

Talia Harris1, Galit Eliyahu2, Lucio Frydman1, Hadassa Degani2

1Chemical Physics, Weizmann Institute of Science, Rehovot, Israel; 2Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

The enhanced polarization enabled by ex situ Dynamic Nuclear Polarization may allow us to follow metabolic processes non-invasively with unprecedented sensitivity and temporal resolution. In order to understand the altered metabolism of cancer and screen for additional biomarkers we have developed a perfusion-infusion bioreactor, allowing hyperpolarized metabolic measurements on living cell cultures. In this work we compare the metabolism of 1-13C-Pyruvate and 1-13C-Lactate in breast cancer cells. The kinetic measurements allow us to demonstrate that the metabolism of 1-13C-Lactate is transport limited, as was previously established for 1-13C-Pyruvate.