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Abstract #1025

A Feasibility Study in Mini-Pig for Heart Metabolism with Hyperpolarized [1-13C]pyruvate: MRS Cardiac Modelling and Kinetic Considerations

Francesca Frijia1, Luca Menichetti1, Vincenzo Positano1, Vincenzo Lionetti2, Claudia Forte3, Jan H. Ardenkjaer-Larsen4, Matteo Milanesi1, Giulio Giovannetti1, Daniele De Marchi1, Giovanni Aquaro1, Manuela Campan2, Fabio A. Recchia5, Luigi Landini1, Maria Filomena Santarelli1, Massimo Lombardi1

1MRI Lab, "G. Monasterio" Foundation and Institute of Clinical Physiology, Pisa, Italy; 2Sector of Medicine, Scuola Superiore Sant'Anna, Pisa, Italy; 3Istituto per i Processi Chimico Fisici, CNR,, Pisa, Italy; 4GE Healthcare, Huginsvej 8, 3400 Hillerod,, Denmark; 5Department of Physiology, New York College, Valhalla, New York

Changes in metabolic products of pyruvate can be correlated to the patho-physiological condition of the myocardium: The cardiac oxidation of pyruvate depends on oxygen delivery to myocardium and on the activation state of mitochondrial pyruvate dehydrogenase. The real time tracking of the metabolic fate of pyruvate in the intact heart with MRS would provide a key information on the state of myocardium in response to a variety of stimuli. This study deal with the real time in vivo cardiac metabolism after intravenous injection of hyperpolarized [1-13C]-pyruvate in the animal of mid size with a 3T scanner with regards to the typical kinetic profile of accumulation of each metabolite and if the typical pattern could be modelled with simple equations.