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Abstract #1028

Effect of Binding on Hyperpolarized MR Signals

Kayvan R. Keshari1, David M. Wilson, Daniel B. Vigneron, Jeffrey M. Macdonald2, John Kurhanewicz

1University of California, San Francisco, San Francisco, Ca, United States; 2University of North Carolina, Chapel Hill

The purpose of this study was to use hyperpolarized 13C-spectroscopy in the benzoic acid-β-cyclodextrin system to understand the relationship between binding and loss of hyperpolarized signal. The apparent T1 relaxation times for the C1 and C2 carbons of benzioc acid decreased in the presence of β-cyclodextrin, and the changes in T1 relaxation with benzoic acid concentration were used to determine the binding constant (log K 1.68-1.74). Hyperpolarized 13C-spectroscopy may have a role in the rapid screening of small molecular weight drug binding constants in vitro and determining the impact of enzymatic binding on hyperpolarized metabolic probe T1s.