Kayvan R. Keshari1, David M. Wilson, Daniel
B. Vigneron, Jeffrey M. Macdonald2, John Kurhanewicz
1University of California, San
Francisco, San Francisco, Ca, United States; 2University of North
Carolina, Chapel Hill
The
purpose of this study was to use hyperpolarized 13C-spectroscopy
in the benzoic acid-β-cyclodextrin system to understand the relationship
between binding and loss of hyperpolarized signal. The apparent T1
relaxation times for the C1 and C2 carbons of benzioc
acid decreased in the presence of β-cyclodextrin, and the changes in T1
relaxation with benzoic acid concentration were used to determine the binding
constant (log K 1.68-1.74).
Hyperpolarized 13C-spectroscopy may have a role in the
rapid screening of small molecular weight drug binding constants in vitro and
determining the impact of enzymatic binding on hyperpolarized metabolic probe
T1s.
Keywords