Valeria Righi1,2, Alex A. Soukas3, Gary Ruvkun3, A Aria Tzika1,2
1NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States; 2Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Athinoula A. Martinos Center for Biomedical Imaging, Boston, MA, United States; 3Department of Genetics, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States
We demonstrate metabolic biomarker profiles with high-resolution magic angle spinning proton MR spectroscopy (HRMAS H1 MRS) of living Caenorhabditis elegans (C. elegans) worms. This work opens up perspectives for the use of H1 HRMAS-MRS as a metabolic profiling method for C. elegans. Because it is amenable to high throughput and is shown to be highly informative, this approach may lead to a functional and integrated metabolomic analytic approach of the small organism C. elegans, which has been used extensively in studies of aberrant metabolism, and should help in identifying, investigating, and even validating new pharmaceutical targets for metabolic diseases.