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Abstract #1046

Visualization of Vascular Casts Using 3D MR Imaging

Ian Rowland1, Joseph Heintz2, Douglas Steeber3, Ralph Albrecht2

1Department of Radiology, University of Wisconsin, Madison, WI, United States; 2Department of Animal Sciences, University of Wisconsin, Madison, WI, United States; 3Department of Biological Sciences, University of Wisconsin, Milwaukee, WI, United States

This study demonstrates that at 4.7T, using a standard 3D gradient echo sequence, images of vascular casts prepared using established corrosion casting techniques may be obtained with an isotropic resolution <50um. Immersion of the casts in aqueous contrast agent enabled 3D representations of the casts to be generated via the absence of MR signal. Despite the lower resolution of MR, surface rendering of the data is able to guide SEM studies to specific regions of interest. Accurate 3D data from vascular casts can be compared with in vivo angiography studies and may also be used to validate direct and indirect methods of measuring vascular dimensions.