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Abstract #1068

A Novel Mitochondrial Peptide Causes Recovery of Skeletal Muscle After Burn Trauma as Assessed with P31 NMR and Electron Paramagnetic Resonance in Vivo

Valeria Righi1,2, Caterina Constantinou, 1,3, Dionyssios Mintzopoulos1,2, Nadeem Khan4, Sriram P. Mupparaju4, Harold M. Swartz4, Hazel H. Szeto5, Ronald G. Tompkins6, Laurence G. Rahme3, A Aria Tzika1,2

1NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States; 2Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Athinoula A. Martinos Center for Biomedical Imaging, Boston, MA, United States; 3Molecular Surgery Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States; 4EPR Center for Viable Systems, Department of Diagnostic Radiology, Dartmouth Medical School, Hanover, NH, United States; 5Department of Pharmacology, Joan and Sanford I. Weill Medical College of Cornell University, New York, NY, United States; 6Department of Surgery, Division of Burn, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States


Using P31 NMR and Electron Paramagnetic Resonance (EPR) in vivo, we evaluated the effects of a novel (Szeto-Schiller) SS-31 peptide on ATP synthesis rate and redox status, respectively in a clinically relevant burn trauma model. Our results showed that SS-31 peptide induces ATP synthesis rate and causes recovery of the mitochondrial redox status at 6 hours after burn. Thus, EPR, can be used to complement NMR in investigating, and even validating novel mitochondrial agents for burn trauma and a variety of pathologies (i.e., stroke, heart failure, diabetes, degenerative disorders, trauma, cancer) associated with mitochondrial dysfunction, including normal aging.