Noam Shemesh1, Evren zarslan2, Peter J. Basser2, Yoram Cohen1
1School of Chemistry, Tel Aviv University, Tel Aviv, Israel; 2Section on Tissue Biophysics and Biomimetics, NICHD, National Institutes of Health, Bethesda, MD, United States
Although single-pulsed-field-gradient (s-PFG) methodologies such as DTI and the q-space approach are widely used to probe tissue microstructures, they suffer from inherent limitations, especially when specimens are characterized by randomly oriented compartments or size distributions. The double-PFG (d-PFG) is emerging as a new probe for novel microstructural information that cannot be achieved by other means. Here we demonstrate that d-PFG can be used to extract accurate compartment dimensions at low q-values both in phantoms and in biological cells which are randomly oriented, and in optic and sciatic nerves. The d-PFG may become an important MRI method in the CNS.