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Abstract #1664

Validation of in Vivo Mouse Brain Fiber Tracking with Correlative Axonal Tracing in Wild-Type and Reeler Animals

Laura-Adela Harsan1, Csaba David2, Marco Reisert1, Susanne Schnell1, Jrgen Hennig1, Dominik von Elverfeldt1, Jochen F. Staiger2

1Department of Diagnostic Radiology, Medical Physics, University Hospital, Freiburg, Germany; 2Department of Neuroanatomy, Institute for Anatomy and Cell Biology, Freiburg, Germany


The present study validates an in-vivo DT-MRI and FT protocol capable of identifying and characterizing the subtle connection pathways in the living mouse brain. The reconstructions of the thalamocortical projections derived from in-vivo DT-MRI were co-registered and correlated with 3D reconstructions of the fibers labeled with Phaseolus vulgaris-leucoagglutinin histological tracer, injected in the thalamus of the same animal. Good agreement between the deterministic and probabilistic tractography and the histological tracing was obtained in wild type and reeler mutant brains

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