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Abstract #1712

Non-Invasive MRI Measurement of CBF: Validating an Arterial Spin Labelling Sequence with 99mTc-HMPAO CBF Autoradiography in a Rat Stroke Model

Tracey Anne Baskerville1, Christopher McCabe1, Jim Patterson2, Juan Chavez3, I Mhairi Macrae1, William M. Holmes1

1Glasgow Experimental MRI Centre, University of Glasgow, Glasgow, Lanarkshire, United Kingdom; 2Institute of Neurological Sciences, Southern General Hospital, Glasgow, United Kingdom; 3Discovery Translational Medicine, Wyeth Research, Collegeville, PA, United States

Arterial spin labelling (ASL) has provided some valuable insight into cerebral perfusion in stroke research. ASL has the advantages of being non-invasive, allows repeated scanning in the same subject and can generate fully quantitative cerebral blood flow (CBF) measurements; however it requires further validation in rodent stroke models. We modified a published ASL technique (Moffat et al, 2005) and validated it against an established autoradiographic technique using the SPECT ligand, 99mTC-D, L-Hexamethylpropyleneamine (99mTc-HMPAO) in a rodent stroke model. We found that relative CBF estimates in cerebral regions of interest generated from ASL and autoradiography were closely matched throught MCA territory and ASL was able to accurately detect reductions in CBF in ischaemic tissue.