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Abstract #1801

Detecting Blood-Brain Barrier Disruption Under Biosafety Regime Using Optimum Transcranial Focused Ultrasound and Improved Contrast-Enhanced MRI

Jun-Cheng Weng1,2, Sheng-Kai Wu3, Win-Li Lin3,4, Wen-Yih Iascc Tseng1,5

1Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine, Taipei, Taiwan; 2Department of Medical Imaging and Radiological Sciences, Chung Shan Medical University, Taichung, Taiwan; 3Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan; 4Medical Engineering Research Division, National Health Research Institutes, Miaoli, Taiwan; 5Department of Medical Imaging, National Taiwan University Hospital, Taipei, Taiwan

Focused ultrasound (FUS) along with an ultrasound contrast agent (UCA) can induce transient and local increase in the permeability of blood vessel wall or cell membrane, and the change in blood-brain barrier (BBB) permeability can be appropriately indicated by contrast-enhanced MRI. Recently, most studies have used optimum FUS parameters with intravascular injection of pre-formed micro-bubbles to produce BBB disruption with minimum damage to the neurons. However, there are no studies reporting that under biosafety regime BBB disruption could still be predicted by MR contrast enhancement. The purpose of this study was to see if the traditional T1-weighted (T1W) imaging sequences, spin echo (SE) and gradient echo (GE), can discern the difference in the BBB disruption in lower dose regime or not. A high sensitivity R1 mapping was used as a gold standard and absolutely quantification. The quantitative analysis indexing the degree of BBB disruption and the correlation against Evans blue (EB) staining were also demonstrated. Our results suggest that, in the absence of hemorrhage, contrast-enhanced T1W gradient echo and spin echo sequence were equally reliable in quantifying the BBB disruption.