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Abstract #1878

Lipid-Coated Iron Oxide: A Versatile, Biocompatible and Multimodal Material for Cellular Imaging

Geralda A.F. van Tilborg1, Willem J.M. Mulder2, Susanne M.A. van der Pol3, Louis van Bloois4, Annette van der Toorn1, Gert Storm4, Helga E. de Vries3, Rick M. Dijkhuizen1

1Image Sciences Institute, University Medical Center Utrecht, Utrecht, Netherlands; 2Translational and Molecular Imaging Institute, , Mount Sinai School of Medicine, New York, United States; 3Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, Netherlands; 4Department of Pharmaceutics, Institute for Pharmaceutical Sciences, Utrecht, Netherlands

In this study we propose a novel lipid-coated fluorescent iron oxide particle for simultaneous magnetic and fluorescent cell labeling. Murine macrophages (RAW) were incubated with the contrast agent and showed efficient labeling without inducing toxicity. Labeled cells were clearly detected with T2-weighted MRI, fluorescence microscopy and flow cytometry. The presented nanoparticulate agent represents a versatile and potent contrast material for cellular imaging, and can be particularly attractive for assessing the fate of in vivo administered labeled cells with multimodal imaging techniques.