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Abstract #1885

In Vivo Detection of CFos-GFP for Neural Activation Via Magnetization Transfer Contrast (MTC) Magnetic Resonance Imaging (MRI)

Carlos J. Perez-Torres1,2, Robia G. Pautler1,2

1Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, United States; 2Department of Molecular Physiology & Biophysics, Baylor College of Medicine, Houston, TX, United States

cFos is a widely utilized marker of neuronal activation that currently requires ex vivo immunohistochemistry for detection. We believe detecting cFos expression in vivo with MRI would provide an unparalleled means to assess neuronal activation in mouse models of human diseases. Importantly, the levels of activation would be a direct measurement of neuronal function. . We have previously described a novel approach to detect Green Fluorescent Protein (GFP) expression in vivo using Magnetization Transfer MRI. Here, we apply the MRI based detection of GFP to evaluate a cFos-GFP fusion model.