Meeting Banner
Abstract #1884

Hyperpolarized 13C MR Reporter Probe System with Acy-I Gene and [1-13C] N-Acetyl-L-Methionine

Albert P. Chen1, Ralph E. Hurd2, Yi-Ping Gu3, David M. Wilson4, Charles H. Cunningham3

1GE Healthcare, Toronto, ON, Canada; 2GE Healthcare, Menlo Park, CA, United States; 3Imaging Research, Sunnybrook Health Sciences Centre, Toronto, ON, Canada; 4Radiology, UCSF, San Francisco, CA, United States

With the recent development of techniques to achieve highly polarized nuclear spins states via dynamic nuclear polarization and retain the polarization in solution, it may be feasible to utilize a suitable hyperpolarized 13C substrate to target an enzyme reporter gene for non-invasive MR imaging of labeled cells/tissue. In this study, a hyperpolarized 13C MR reporter system is demonstrated in cells transfected to overexpress a reporter gene, aminoacylase I (Acy-I), using pre-polarized [1-13C] N-acetyl-L-methionine as the reporter probe.