Anne H. Schmieder2, Samuel A. Wickline2, Gregory M.
Lanza2, Shelton D. Caruthers2
1Philips Research Europe, Hamburg,
Germany; 2C-TRAIN, Washington University, St. Louis, MO, United
stratification using molecular MRI of angiogenesis could change standard of
care in anti-angiogenic therapy. Previously, α ν β
3-integrin targeted nanoparticles (NP) have been shown to detect and
quantify angiogenesis in small-animal tumor models based on 19F-MRI.
These promising results using Perfluoro-Crown-Ether labels are currently
translated to more clinically-relevant Perfluoro-Octyl-Bromide (PFOB) NP. The
complex spectral properties of PFOB and the sensitivity to the target-binding
process, as observed in this work, require a thorough optimization of imaging
parameters on target. In vitro
optimization on fibrin clots and in
vivo detection of angiogenesis-targeted NP in the vasculature of
Vx2-tumor bearing rabbits by 19F-MRI is demonstrated.