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Abstract #1936

MR Molecular Imaging of HER-2 in a Murine Tumor Xenograft by SPIO Labeling of Anti-HER-2 Affibody.

Manabu Kinoshita1, Yoshichika Yoshioka2, Yoshiko Okita1, Naoya Hashimoto1, Toshiki Yoshimine1

1Department of Neurosurgery, Osaka University Medical School, Suita, Osaka, Japan; 2High Performance Bioimaging Research Facility, Osaka University, Graduate School of Frontier Biosciences, Suita, Osaka, Japan

In vivo molecular imaging is a rapidly growing research area both for basic and clinical science. Non-invasive imaging of in vivo conditions in a molecular level will help understand the biological characteristics of normal and diseased tissues without performing surgical invasive procedures. Among various imaging modalities, magnetic resonance imaging (MRI) has gained interest as a molecular imaging modality for its high special resolution. In this research, we have demonstrated that the combined use of HER-2 targeting Affibody, a small 7kDa molecule that behaves similarly to antibodies, and superparamagnetic iron oxide (SPIO) can non-invasively image HER-2 expressing cells or tissues both in vitro and in vivo by MRI. This preliminary study demonstrates that Affibody-SPIO is a feasible target specific contrast agent for in vivo MR-molecular imaging.