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Abstract #1972

1H NMR Metabolomics Study of Cerebrospinal Fluid (CSF) in Amyotrophic Lateral Sclerosis (ALS) Patients

Lydie Nadal-Desbarats1, Helne Blasco2, Segolene Veau2, Patrick Vourc'h2, Caroline Moreau3, David Devos3, Philippe Corcia4, Christian R. Andres2

1Laboratoire de RMN, INSERM U930 - CNRS 2448 - Universit Franois Rabelais, Tours, France; 2Laboratoire de Biochimie et Biologie moleculaire, Inserm U930-CNRS 2448 - Universit Franois Rabelais, Tours, France; 3Service de Neurologie et Pathologie du Mouvement, EA2683, Hopital R. Salengro - CHRU Lille, Lille, France; 4Centre SLA, CHRU Bretonneau, Tours, France

Amyotrophic lateral sclerosis is a progressive neurodegenerative disease. Pathophysiological mechanisms involved in this disease are complex but remain for the most part unknown. This lack of knowledge might explain the absence of reliable biological marker. CSF could be a source of biomarkers. The aim of this study was to analyze CSF of patients with ALS by 1H NMR in order to identify biomarkers in the early stage of the disease, and to evaluate the biochemical factors involved in this disease. We quantified 18 metabolites like amino-acids, organic acids and ketonic bodies. Higher concentrations of metabolites such as ketone bodies contribute to the PCA separation between the two populations.