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Abstract #2387

NMR Investigations of Excitatory and Inhibitory Neurotransmission in Mouse Brain

Anant Bahadur Patel1, Vivek Tiwari1, A.L. Susmitha1, K.S. Varadarajan1

1NMR Microimaging and Spectroscopy, Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India

Knowledge of neurotransmitter metabolism is very important for understanding the pathophysiology of neurological disorders. In the present study we have investigated neuronal TCA cycle and neurotransmitter cycle flux in different brain regions of C57BL6 mouse. Mice were infused with [1,6-13C2]glucose for different time ranging from 7 to 90 min or [2-13C]acetate for ~90 min. Brain metabolite levels and 13C labeling of amino acids were measured with 1H-[13C]-NMR spectroscopy at 14T NMR spectrometer. The metabolite levels were distinct in different regions of the brain. Glutamatergic rate was higher in cortex while GABAergic was more in cerebellum and olfactory bulb.