Dong-Cheol Woo1, Chi-Bong Choi2,
Sung-Ho Lee3, Eunjung Bang4, Sang-Soo Kim1,
Hyang-Shuk Rhim1, Sang-Young Kim1, Bo-Young Choe1
1The Catholic University of Korea,
Seoul, Korea, Republic of; 2Kyung-Hee University of Korea; 3Konkuk
university of Korea; 4Korea Basic Science Institute
This
study was to characterize the regional neurochemical profiles of canine brain
using NMRS, tissue extraction, and external simulated phantom concentration
quantification. The occipital, frontal, and temporal lobes, thalami,
cerebellar cortices, and spinal cords of adult beagles were obtained, and NMR
samples were prepared using M/C extraction method. The metabolite
concentrations in canine brain tissues were measured and compared with those
found in human and rat brain. In addition, the cross peaks of brain
metabolites were identified using 2D-COSY. This study demonstrated the
absolute quantification of canine neuronal parts using MRS, with tissue
extraction used to measure metabolite concentrations.
Keywords