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Abstract #2549

Quantitative Assessment of Lung Microstructure in Healthy Mice Using Lung Morphometry with Hyperpolarized 3He Diffusion MRI

Emir Osmanagic1,2, Alexander L. Sukstanskii3, James D. Quirk3, Jason C. Woods3,4, Mark S. Conradi4, Dmitriy A. Yablonskiy3,5

1Electrical and Systems Engineering, Washington University, St. Louis, Misssouri, United States; 2Radiology, Washington University, St. Louis, MO, United States; 3Radiology, Washington University, St. Louis, Misssouri, United States; 4Physics, Washington University, St. Louis, MO, United States; 5Physics, Washington University, St. Louis, Misssouri, United States


Lung morphometry technique with hyperpolarized 3He allows quantification of lung geometrical parameters such as mean chord length Lm, surface-to-volume ratio S/V and density of alveoli. It was demonstrated that in humans, it provides results similar to direct morphological measurements. Two important modifications, however, are required to adopt this technique for studying lung microstructure in small animals reduction in diffusion time and modification of theoretical relationship between diffusion MR signal and lung microstructural parameters. Herein we provided such modifications and demonstrated that measurements obtained with lung morphometry with hyperpolarized 3He MRI in mice are in agreement with literature data.

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