Valeria
Righi1,2, Yiorgos Apidianakis3, Dionyssios Mintzopoulos1,2,
Loukas G. Astrakas, 1,4, Laurence G. Rahme3, A Aria
Tzika1,2
1NMR Surgical Laboratory, Department of
Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard
Medical School, Boston, MA, United States; 2Department of
Radiology, Massachusetts General Hospital, Harvard Medical School, Athinoula
A. Martinos Center for Biomedical Imaging, Boston, MA, United States; 3Molecular
Surgery Laboratory, Department of Surgery, Massachusetts General Hospital and
Shriners Burns Institute, Harvard Medical School, Boston, MA, United States; 4Department
of Medical Physics, University of Ioannina, Ioannina, Greece
We
demonstrate biomarker profiles with high-resolution magic angle spinning
proton MR spectroscopy (HRMAS H1 MRS) of live Drosophila melanogaster flies.
We show that the metabolic HRMAS MRS profiles of adipokinetic hormone
receptor (akhrnull) mutant flies, which have an obesity phenotype, are
different from isogenic control strain flies (akhrrev). Our approach advances
the development of novel, in vivo, non-destructive research approaches in
Drosophila, suggests biomarkers for investigation of biomedical paradigms,
and thus may contribute to novel therapeutic development in obesity.